Objectives of Programme's Learning Outcomes

• In the field of biological sciences and particularly in the field of the biology of organisms and ecology, possess highly specialised and integrated knowledge and a wide range of skills adding to those covered in the Bachelor's programme in biological sciences.
• Manage and lead research, development and innovation projects.
• Organise and lead a research, development or innovation project to completion.
• Develop and integrate a high degree of autonomy.
• Pursue further training and develop new skills independently.
• Apply scientific methodology.
• Demonstrate thoroughness, independence, creativity, intellectual honesty, and ethical values.

Learning Outcomes of UE

The purpose of this course is to provide information on the practice of light and electron microscopy and the preparation of cells and tissues for microscopic examination with emphasis on immunohistochemistry and morphometry

 

UE Content: description and pedagogical relevance

The first part of the course is concerned with instrumentation (optical microscope) and the methods used to obtain informative images. The second part is devoted to traditional preparatory techniques for light and electron microscopy including fixation, sectioning and staining as well as histochemistry. The third section covers the various aspects of immunohisochemistry such as fixation and tissue preparation, antigen retrieval techniques, multiple immunostaining. The last section is an introduction to computer assisted-morphometry applied to histological sections.
The course is given entirely in French.

Prior Experience

Not applicable